Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 07/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | Ladybug |
| Putative identification | Arthropoda Hexapoda Insecta Coleoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | some of the bands did not show up or were unclear including the arthropod bands and the seventh band of the wolbachia gel |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Coleoptera was found to be negative for Wolbachia. |
Water Boatman