JSEZ1

Sample information

Picture
Photos by: Jaylin S
Location
Collection date 07/01/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

Ladybug

Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results

Wolbachia presence No
Confidence level Low
Explanation of confidence level

some of the bands did not show up or were unclear including the arthropod bands and the seventh band of the wolbachia gel

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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