JSEZ1

May 15, 2023

Sample information
Picture
Photos by: Jaylin S
Location
Collection date 07/01/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

Ladybug
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

some of the bands did not show up or were unclear including the arthropod bands and the seventh band of the wolbachia gel
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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