T4P143210 (centipede)

May 15, 2023

Sample information
Picture
Photos by: Angela M., Crystal N., Lynda T., Allen A.
Location
Collection date 04/07/2023
Captive / Cultivated? Wild-caught
Group Arroyo High School
Observations

-Damp location

-Ground was Dirt

-Found next to a slug

-Found under a rock
Putative identification Arthropoda Myriapoda Chilopoda

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Rear half
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

We obtained the centipede by lifting a rock in an unbothered area that happened to hide the insect and simply retrieved it into a container.

Results
Wolbachia presence Unknown
Confidence level Low
Explanation of confidence level

Something must have gone wrong during the gel electrophoresis process. Our Wolbachia gel has DNA but it’s smeared all over the top of the gel. We did not place our gel in the wrong orientation, running the gel from negative to positive, yet we still had this occur. As to right now, we have no clue on why this occured.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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