Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 04/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Arroyo High School |
| Observations | -Damp location -Ground was Dirt -Found next to a slug -Found under a rock |
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | We obtained the centipede by lifting a rock in an unbothered area that happened to hide the insect and simply retrieved it into a container. |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | Something must have gone wrong during the gel electrophoresis process. Our Wolbachia gel has DNA but it’s smeared all over the top of the gel. We did not place our gel in the wrong orientation, running the gel from negative to positive, yet we still had this occur. As to right now, we have no clue on why this occured. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Water Boatman