Pingry Bugs and Bacteria – Debra Hill, Ellie Solomon, Keira Troy

June 8, 2023

Sample information
Picture
Photos by: Debra H
Location
Collection date 05/30/2023
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

The Leucauge venusta spider is distinctly colored with leaf-green legs. The abdomen is shiny capsule-shaped and tubular. Yellow markings appear on the abdomen and black blotches are on the end of it. There is a straight black line with smaller lines extending from the midline down the center of the abdomen. The legs are green with black bands at the joints. On the rear of the abdomen, there is a small red spot.
Putative identification Arthropoda Chelicerata Arachnida Araneae Tetragnathidae Leucauge Leucauge venusta

Methods
Extraction kit DNeasy (Qiagen) blood + tissue kit with modification P2 for ATL + N3 for AL
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

The confidence alignment level is high because Wolbachia identifies 100% in the 416 base pairs.
Wolbachia 16S sequence Download FASTA   
GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGA GCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTG GGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGT CGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGC TAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT TANNNTCGAAGCTA
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA   
TTTTNNGTGCTTGAGCTTCATAATAGGGACAGCTATAAGTGTGCTAATTCCGATTGAGTTACGTCAA TCGGGAAGATTTTTAGGCAATGATCAATTGTATAATGTGATTGTAACAGCTCATGCTTTTATTATAATTTTTTTTATAAT TATACCTATTTTAATTGGGGGATTTGGAAATTGATTGGTTCCTTTAATATTAGGGGCTCCTGATATGGCTTTTCCTCGCA TAAATAATTTAAGTTTCTGACTATTACCTCCTTCACTCTTTTTATTATTTATTTCTTCAATGGTAGATATANGAGTGGGG GCTGGGTGAACTGTATATCCTCCTTTAGCTTCTTTAAAAGGGCATTCTGGTAATTCTATGGATTTTGCTATTTTTTCTCT TCATTTAGCTGGGGCTTCTTCTATTATAGGGGCTATTAATTTTATTTCTACTATTTTTAATATACGGGTAAATGGAATAA AAATATAAAAATTTCCTTTATTTGTTTGATCTGTGTTAATTACNNNTGTTTTATTATTATTATCCCTCCCGGTTTNGGCN NGGGCNANTACTATATTGNTAACANATCGCANTTTTAATACTTCTTTTTNTGACCCTTCTGGANGANGGGATCCAGTTCT TTTTCCACATTNATTTTNATTTTTNGGNNNNNNNANNTTAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Leucauge venusta was found to be postive for Wolbachia.
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