We accidentally used ladder instead of loading dye when we were preparing our samples, and as a result, our gel was a little hard to read. However, the ladder solution had loading dye in it, so the DNA still ran through the gel. We re-ran the whole process, but our new gel ended up having no results, not even in our controls, so we kept this gel.

The sample extracted from the Leucauge venusta is in lane 2 (shown in the table). We prepped the samples and then ran them through a gel for about 15 minutes at 100 watts. The top half of the gel shows results for the C01 arthropod gene, and the bottom half shows results for the 16s Wolbachia gene. The sample in lane 2 had a band aligned with the band from the positive arthropod control, showing a positive result for the CO1 arthropod gene for the spider. The sample in lane 2 also had a band that lined up with the positive Wolbachia control, meaning the spider had the 16S Wolbachia gene in addition to the arthropod CO1 gene.