Asian lady beetle- Uma M and Carolyn Z

June 7, 2023

Sample information
Picture
Photos by: Carolyn Z
Location
Collection date 05/30/2023
Captive / Cultivated? Wild-caught
Group Pingry School
Observations

Reddish orange wings with black spots. White M shape on head with small black spots. Rounded oval like dome, two antennas and 6 small legs. Black inner body covered by wings.
Putative identification Arthropoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

1% Agarose gel set at 140 for approximately 30 minutes.

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

Gel had faint band for Wolbachia, no conclusive matches from database of Wolbachia sequence.
Wolbachia 16S sequence
Faint gel, no match
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
GGAATAGTAGGAACATCGTTAAGTATTTTAATTCGGTTAGAATTAGGAACTAGAGGAAGATTAATTGGAAACGACCAAATTTATAATATAATTGTTACAGCTCATGCTTTCATTATAATTTTCTTTATAGTAATACCTATTATAATTGGGGGTTTTGGAAATTGATTAGTTCCTTTAATAATTGGAGCTCCTGATATAGCATTTCCACGATTAAATAACATAAGATTTTGACTTTTACCCCCTGCTTTAACTCTTTTAATTTTAAGAACAATCGTAGAAATAGGGGCAGGAACAGGATGAACTGTTTACCCTCCTCTTTCTTCTAATTTAACACATAATGGGCCTTCAGTAGATTTAGTGATTTTTAGTTTACATTTAGCAGGAATTTCCTCAATTTTAGGTGCAGTAAATTTCATTTCAACTATTATAAATATACGTCCATTTGGTATAATACTTGATAAAACTCCTTTATTTGTATGATCTGTTCTTATTACAGCAATTCTTTTATTACTATCACTACCAGTTCTTGCAGGAGCAATTACTATACTATTAACTGACCGAAACTTAAATTCTTCTTTTTTTGACCCAACCGGTGGGGGAGACCCAATTTTATACCAACAGTTATT
BLAST at The Wolbachia Project   BLAST at NCBI
Summary
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