Triangulate Cobweb Spider- Lauren (LP01)

Sample information
Picture	Photos by: Myself
Location
Collection date	05/30/2023
Captive / Cultivated?	Wild-caught
Group	Pingry School
Observations	Fast and jumpy
Putative identification	Arthropoda Chelicerata Arachnida Araneae Theridiidae Steatoda Steatoda triangulosa
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Whole arthropod
Single or Duplex PCR	Single Reaction
Gel electrophoresis system	Standard electrophoresis system
Buffer	TAE
DNA stain	SYBR Safe
Gel images
Protocol notes
Results
Wolbachia presence	Yes
Confidence level	High
Explanation of confidence level	100% same sequence to 16s sequence
Wolbachia 16S sequence	Download FASTA Download AB1 GGTGTTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGA GCGCAACCCTCATCCTTAGTTACCATCAGGTAATGCTGGGGACTTTAAGGAAACTGCCAGTGATAAACTGGAGGAAGGTG GGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGT CGCGAGGCTAAGCCAATCCCTTAAAAGCCATCTCAGTTCGGATTGTACTCTGCAACTCGAGTGCATGAAGTTGGAATCGC TAGTAATCGTGGATCAGCACGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGT TTCACTCGAAG BLAST at The Wolbachia Project BLAST at NCBI
Arthropod COI sequence	Unsuccessful PCR- see bottom half of gel BLAST at The Wolbachia Project BLAST at NCBI
Summary	The Steatoda triangulosa was found to be postive for Wolbachia.

The Wolbachia Project Database