Thrive Scholars – Summer 2023 – Ant-Butterflywasps

Sample information

Picture
Location
Collection date 08/07/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website.

Results

Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

The gel image was hard to read. However I do think it is wolbachia positive because we do see a park  which can possibly be the wolbachia. This sample is in the third lane.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be postive for Wolbachia.
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