TheBlueGloves-Pill Bug

August 9, 2023

Sample information
Picture
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations

The pill bug was small with a hard outer shell. The insect’s color was a light grayish-brown. It was difficult to sacrifice.
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website

Results
Wolbachia presence Yes
Confidence level Low
Explanation of confidence level

Some of our gel samples were smeared. However there was a good positive Wolbachia placement between the 400 and 500 bands in the third column
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be postive for Wolbachia.
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