Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/08/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Thrive Scholars |
| Observations | The pill bug was small with a hard outer shell. The insect’s color was a light grayish-brown. It was difficult to sacrifice. |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | We followed the protocol on the website |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | Low |
| Explanation of confidence level | Some of our gel samples were smeared. However there was a good positive Wolbachia placement between the 400 and 500 bands in the third column |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidae was found to be postive for Wolbachia. |
Melanoplus bruneri
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata