Thrive Scholars – Summer 2023 – Ant – Easties

Sample information

Picture
Location
Collection date 08/08/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Observations
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods

Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website.

the top gel is wolbachia

bottom gel is arthropod

The sample is in third lane of both gels

Results

Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

The controls didn’t work. There was no Arthopod and Wolbachia.

Wolbachia 16S sequence
Arthropod COI sequence
Summary
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