Thrive Scholars – Summer 2023 – Pillbug – ECB

Sample information

Collection date 08/07/2023
Captive / Cultivated? Wild-caught
Group Thrive Scholars
Putative identification Arthropoda Crustacea Malacostraca Isopoda


Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain GelRed
Gel images
Protocol notes

We followed the protocol on the website.

Top gel is Arthropod gel.

Bottom gel is Wolbachia gel.

Sample is in Lane two of gel one and lane four of gel two

We might have swapped the samples.



Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

We suspect that the control samples were swapped which caused the bands to be off.

Wolbachia 16S sequence
Arthropod COI sequence
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