Black Carpenter Ant

September 11, 2023

Sample information
Picture
Photos by: Veronica T.
Location
Collection date 09/24/2020
Captive / Cultivated? Wild-caught
Group Hillsboro High School
Observations

Was caught attached to a Red CarpenterAnt and was later detached. Was found at the base of a tree with other ants nearby.
Putative identification Arthropoda Hexapoda Insecta Hymenoptera Formicidae

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Whole arthropod
Single or Duplex PCR
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident with the controls and protocol, and the band on the gel was thick.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Formicidae was found to be postive for Wolbachia.
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