Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/07/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Thrive Scholars |
| Observations | |
| Putative identification | Arthropoda Hexapoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | We followed the protocol on the website. Top gel is Arthropod gel. Bottom gel is Wolbachia gel. The sample is in lane 3 for the top gel The sample is in lane five for the bottom gel We might have swapped the samples.
|
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | We suspect that the control samples were swapped which caused the bands to be off. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Spotted cucumber beetle (Diabrotica undecimpunctata) C-5 SSP
DKH10
DKH 9
DKH8
DKH 7