Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/10/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Portland State University |
| Observations | This insect was found on a milkweed plant in an urban garden. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Chlorochroa |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Partial abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | GelRed |
| Gel images |
|
| Protocol notes | We use GoTaq Green Master Mix, 1% agarose gel, 1% TAE buffer, and 95 V for one hour. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | This was part of a protocol test. I extracted the DNA from this stink bug on the same day with the same protocol as two positive control and two negative control Drosophila supplied by your project. I dissected two pieces of abdomen from the stink bug and ran through the extraction protocol with both. Neither of the stink bug DNA extracts gave positive results for Wolbachia while both of the positive control Drosophila did. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Chlorochroa was found to be negative for Wolbachia. |
Water Boatman