|Captive / Cultivated?
|Portland State University
This insect was found on a milkweed plant in an urban garden.
|Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Chlorochroa
|DNeasy (Qiagen) blood and tissue kit
|DNA extraction location
|Single or Duplex PCR
|Gel electrophoresis system
|Standard electrophoresis system
We use GoTaq Green Master Mix, 1% agarose gel, 1% TAE buffer, and 95 V for one hour.
|Explanation of confidence level
This was part of a protocol test. I extracted the DNA from this stink bug on the same day with the same protocol as two positive control and two negative control Drosophila supplied by your project. I dissected two pieces of abdomen from the stink bug and ran through the extraction protocol with both. Neither of the stink bug DNA extracts gave positive results for Wolbachia while both of the positive control Drosophila did.
|Wolbachia 16S sequence
|Arthropod COI sequence
|The Chlorochroa was found to be negative for Wolbachia.