Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/28/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | Found under a rock on a middle school playground in late summer (78 degrees F). Urban habitat. The bug is dark yellow and grey; its body is 3 parts and it has wings. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | |
| DNA stain | |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be negative for Wolbachia. |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus