Firefly

September 28, 2023

Sample information
Picture
Photos by: Iyana C
Location
Collection date 09/10/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

The arthropod has a red head with a black spot, black wings, and 6 legs. The environment was a clear grassy yard.
Putative identification Arthropoda Hexapoda Insecta Coleoptera Lampyridae Photinus Photinus pyralis

Methods
Extraction kit promega wizard SV
DNA extraction location
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

We may have accidentally washed out our dna.

Results
Wolbachia presence Unknown
Confidence level High
Explanation of confidence level

We’re unsure about the results because no bands showed up for anything.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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