Arachnid

September 28, 2023

Sample information
Picture
Photos by: Leonardo G
Location
Collection date 09/11/2023
Captive / Cultivated? Wild-caught
Group Antioch High School
Observations

Green, small deceased.
Putative identification Arthropoda Arachnida Araneae Araneidae

Methods
Extraction kit promega wizard SV
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TBE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Medium
Explanation of confidence level

I was a bit too unsure about if we had Wolbachia but turned out we didn’t, and I expected that a little.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Araneidae was found to be negative for Wolbachia.
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