Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/11/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Antioch High School |
| Observations | Green, small deceased. |
| Putative identification | Arthropoda Chelicerata Arachnida Araneae Araneidae |
Methods |
|
| Extraction kit | promega wizard SV |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes |
|
Results |
|
| Wolbachia presence | No |
| Confidence level | Medium |
| Explanation of confidence level | I was a bit too unsure about if we had Wolbachia but turned out we didn’t, and I expected that a little. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Araneidae was found to be negative for Wolbachia. |
Water Boatman