Allonemobius fasciatus (F-4)

November 20, 2023

Sample information
Picture
Photos by: Charlie L.
Location
Collection date 09/15/2023
Captive / Cultivated? Wild-caught
Group Edmund Burke School
Observations

Dark green coloring on the top of the body yellow covering the bottom. Two antennae, long and black. Long bendable legs with little spikes toward the
bottom of the legs
Putative identification Arthropoda Hexapoda Insecta Orthoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system MiniOne
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

The used pippet tip went into liquid but the used tip was not used, just submerged in the liquid. It was used twice with non-DNA sample liquids.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

No steps were messed up to a degree that would possibly contaminate the DNA
Wolbachia 16S sequence
Arthropod COI sequence Download FASTA    Download AB1
Summary The Orthoptera was found to be negative for Wolbachia.
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