Heidegrashüpfer (Grasshopper, 010)

Sample information

Picture
Location
Collection date 09/28/2020
Captive / Cultivated? Wild-caught
Group Unterstrass
Observations
  • Collected from dry, short grass in a meadow
  • Autumn
  • 15°C
  • Elevation – 571
Putative identification Hexapoda Insecta Orthoptera

Methods

Extraction kit Wizard SV (Promega)
DNA extraction location
Gel electrophoresis system
Buffer
DNA stain
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level
Wolbachia 16S sequence Download FASTA    Download AB1
GCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCAGCGACGAGCGGGCCCTCATCCTTAGATACCATCACATAATGCTGGGGGACTTTAAGGGCACGGGCCTTTGACAATTTTTTTACATATTGCCGGGCATCGCGCACGGGGGGCACCTTCAAATTTTGTGTTGCCCTCATCTTATTCGGGGAGCCTCAGCGCATGAG
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
ATGAGCTGGATAGTAGGTACATCAATAAGAATAATTATTCGTGCAGAACTTGGACAACCAGGATCCATAATTGGAGATGATCAAATCTATAATGTTATTATTACAGCACATGCATTTGTAATAATTTTCTTCATAGTAATACCTATTATAATTGGGGGATTCGGTAATTGACTGGTTCCACTAATAATCGGAGCACCAGATATAGCTTTTCCACGAATAAATAACATAAGTTTTTGACTTTTACCACCATCATTAACTCTTTTAATTGCATCATCAATAGTAGATAATGGTGCAGGAACAGGATGAACAGTTTATCCCCCTCTCGCAGGAGCAATCGCACATGGAGGAGGATCAGTAGACCTGGCTATTTTTTCATTACATTTAGCAGGTGTTTCATCAATTTTAGGAGCAGTTAATTTCATTACAACTGCAATCAATATACGGTCGGAAAGAATAACACTAGATCAAACACCACTATTTGTCTGATCAGTAGCAATTACAGCACTCCTATTACTATTATCATTACCGGTACTAGCAGGAGCAATTACTATATTATTAACTGATCGAAATTTAAATACTTCGTTCTTTGACCCCGCAGGTGGTGGAGATCCAATCCTTTATCAACATTTATTTTGATTTTTTGGTCACCTGGAG
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Orthoptera was found to be postive for Wolbachia.
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