Sample information |
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| Picture |
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|---|---|
| Location | |
| Collection date | 09/28/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | Unterstrass |
| Observations |
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| Putative identification | Arthropoda Hexapoda Insecta Hemiptera Pentatomidae Nezara Nezara viridula |
Methods |
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| Extraction kit | Wizard SV (Promega) |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | |
| Buffer | |
| DNA stain | |
| Gel images |
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| Protocol notes | |
Results |
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| Wolbachia presence | No |
| Confidence level | |
| Explanation of confidence level | |
| Wolbachia 16S sequence | |
| Arthropod COI sequence | Download FASTA
Download AB1
GGTATATGAGCAGGAATAGTAGGATCAGCAATAAGATTAATTATTCGAATTGAACTAGGACAACCCGGAAGATTTATTGGAGATGATCAAATTTATAATGTAGTAGTAACAGCTCACGCATTTGTAATAATTTTCTTTATAGTAATACCAATCATAATTGGAGGATTTGGAAATTGATTAGTACCTTTAATAATTGGTGCTCCAGATATAGCATTCCCTCGAATAAACAATATAAGATTCTGGTTATTACCCCCATCATTAACCCTTTTAATAGTAAGAAGATTAGCAGAATCTGGAGCAGGAACAGGATGAACTGTTTATCCTCCTTTATCTAGTAATTTATCTCATAGAGGAGCCTCAGTGGATTTAGCTATTTTTTCATTACATCTAGCAGGAGTATCATCAATCTTAGGCGCAGTAAATTTCATTTCAACTATTATTAATATACGACCAACAGGTATAACTCCAGAACGAGTTCCACTATTTGTTTGATCAGTTGGGATCACAGCATTATTATTACTACTTTCATTACCTGTACTAGCAGGTGCAATTACAATATTATTAACAGATCGGAACTTTAATACATCATTCTTTGACCCTTCAGGAGGGGGAGATCCCATTCTTTATCAACACTTATTTTGATTTTTTGGTCACCTGAG
BLAST at The Wolbachia Project BLAST at NCBI
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| Summary | The Nezara viridula was found to be negative for Wolbachia. |
Water Boatman