Roley Poley

Sample information

Picture
Location
Collection date
Captive / Cultivated? Wild-caught
Group St. Paul Academy and Summit School
Observations
Putative identification Arthropoda Crustacea Malacostraca Isopoda Armadillidae

Methods

Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniPCR
Buffer TBE
DNA stain GelGreen
Gel images
Protocol notes

Results

Wolbachia presence Yes
Confidence level High
Explanation of confidence level

My teacher would never let me mess up. All controls worked. Was done with advanced equipment and precise technique.

Wolbachia 16S sequence
Arthropod COI sequence
Summary The Armadillidae was found to be postive for Wolbachia.
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