Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/29/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | St. Paul Academy and Summit School |
| Observations | |
| Putative identification | Arthropoda Crustacea Malacostraca Isopoda Armadillidae |
Methods |
|
| Extraction kit | DNeasy (Qiagen) blood and tissue kit |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | My teacher would never let me mess up. All controls worked. Was done with advanced equipment and precise technique. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Armadillidae was found to be postive for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)