Portland State University – Tipulidae

October 31, 2023

Sample information
Picture
Location
Collection date 10/09/2023
Captive / Cultivated? Wild-caught
Group Portland State University
Observations
Putative identification Arthropoda Hexapoda Insecta Diptera Tipulidae

Methods
Extraction kit DNeasy (Qiagen) blood and tissue kit
DNA extraction location Partial abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain GelRed
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Lane 2 shows positive for COI, Lane 3 is a Wolbachia positive control, Lane 4 is blank for Wolbachia in the insect.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Tipulidae was found to be negative for Wolbachia.
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