BROACCF23MS Diptera

November 6, 2023

Sample information
Picture
Photos by: Meghan S.
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection method sweep net, forceps

Habitat tall grasses and weeds along fenceline

Weather conditions raining humid hot
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Controls were as expected

Insect C01 gene prevalent
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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