BroACC_F23_RMP

November 6, 2023

Sample information
Picture
Photos by: Rachael P
Location No map location data available.
Collection date
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Beat stick and canvas,Garden in Armstrong Parking lot,1:40 pm, overcast/light rain,8/21/23
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

There was no cross contamination when we did out gel electrophoresis. All controls worked like how they were supposed to.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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