Sample information |
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Picture |
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Location | No map location data available. |
Collection date | |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations | Beat stick and canvas,Garden in Armstrong Parking lot,1:40 pm, overcast/light rain,8/21/23 |
Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
Extraction kit | |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
|
Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
Wolbachia presence | No |
Confidence level | High |
Explanation of confidence level | There was no cross contamination when we did out gel electrophoresis. All controls worked like how they were supposed to. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Hemiptera was found to be negative for Wolbachia. |