ADG (Hemiptera)

November 6, 2023

Sample information
Picture
Photos by: Alayah Grant
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Method: beat stick & canvas

Habitat: the garden

Weather condition: rainy
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

There were 2 bands for the positive control, 1 band for the negative control, and zero bands for the NTC.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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