Sample information |
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Picture |
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Location | |
Collection date | 11/01/2023 |
Captive / Cultivated? | Wild-caught |
Group | John Overton High School |
Observations | The organism was found under a rock (1 ft wide by 5 inches Long). It was around 30 degrees outside. There were no other animals near it. |
Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
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Extraction kit | DNeasy (Qiagen) |
DNA extraction location | Rear half |
Single or Duplex PCR | Duplex Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | SYBR Safe |
Gel images |
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Protocol notes | |
Results |
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Wolbachia presence | Unknown |
Confidence level | Low |
Explanation of confidence level | The confidence is low because or arthropods CO1 band did not show up. There would have been a mistake done when doing the DNA extraction. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
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Summary |