Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | The organism was found under a rock (1 ft wide by 5 inches Long). It was around 30 degrees outside. There were no other animals near it. |
| Putative identification | Arthropoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Rear half |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | Low |
| Explanation of confidence level | The confidence is low because or arthropods CO1 band did not show up. There would have been a mistake done when doing the DNA extraction. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Agelenopis
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