Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 11/01/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | John Overton High School |
| Observations | What was observed when the insect was collected was that it was next to a tree, under a rock, and there very cold weather that day. |
| Putative identification | Arthropoda Myriapoda Chilopoda |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Duplex Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | – |
Results |
|
| Wolbachia presence | Unknown |
| Confidence level | High |
| Explanation of confidence level | The results that were collected were not visible after being in the transilluminator. The PCR process went well but in the DNA extraction, a step was done wrong. With this information I am confident that the results are unknown. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | |
Pterostichus permundus
Melanoplus Spretus
Centipede- Lithobius forficatus
Fly- Pollen pediculata
Cricket- Acheta domesticus