Horned passalus beetle – Odontotaenius disjunctus

November 7, 2023

Sample information
Picture
Photos by: Pax G.
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations

The arthropod was found underneath a large fallen tree about 10 yards off the trail at Long Hunter State Park.
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence No
Confidence level Low
Explanation of confidence level

The band that confirmed it was an arthropod wasn’t present, so there can’t be full confidence that the negative Wolbachia result is correct.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Coleoptera was found to be negative for Wolbachia.
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