LOGACG23-OL

November 7, 2023

Sample information
Picture
Photos by: Olivia L.
Location
Collection date
Captive / Cultivated? Captive / Cultivated
Group Georgia Southern University
Observations

Caught 8/21/2023 at 6:10 pm in swamp water with moss over it. D-net used for insect collection in an aquatic habitat.
Putative identification Arthropoda Hexapoda Insecta Odonata

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I am confident in my results that my insect tested negative for Wolbachia. Locally, insects of the Odonata order only test positive 20% of the time, which is below average.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Odonata was found to be negative for Wolbachia.
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