Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | |
| Captive / Cultivated? | Captive / Cultivated |
| Group | Georgia Southern University |
| Observations | Caught 8/21/2023 at 6:10 pm in swamp water with moss over it. D-net used for insect collection in an aquatic habitat. |
| Putative identification | Arthropoda Insecta Odonata |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | I am confident in my results that my insect tested negative for Wolbachia. Locally, insects of the Odonata order only test positive 20% of the time, which is below average. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Odonata was found to be negative for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid