Tree Borer Beetle

November 7, 2023

Sample information
Picture
Location
Collection date 11/01/2023
Captive / Cultivated? Wild-caught
Group John Overton High School
Observations
  • underneath a dry tree stump
  • black
  • small
Putative identification Arthropoda Hexapoda Insecta Coleoptera

Methods
Extraction kit DNeasy (Qiagen)
DNA extraction location Abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Unknown
Confidence level Medium
Explanation of confidence level

My confidence level would be a good medium.

One of our controls did work and that was the (+) Arthropod, but the rest were inconclusive after a mishap in the DNA extraction. With that if I had known more about what went wrong during the DNA extraction I could trust the results better than do now.
Wolbachia 16S sequence
Arthropod COI sequence
Summary
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