Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | The insect that I collected was found in behind the greenhouse and it had landed on my hand. I was willing to find more insects but they were being stubborn and it was almost as if the bug wanted to be caught! |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I was confident throughout the process of this project that my insect would be infected with Wolbachia due to the known key facts where my insects habitat is as well as what it intakes. It is common for “love bugs” to be present with Wolbachia. The controls did work in the process and I can definitely trust my results. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be postive for Wolbachia. |
Water Boatman