MACACDF23-25 Hemiptera

November 7, 2023

Sample information
Picture
Photos by: Taryn B.
Location
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

The insect that I collected was found in behind the greenhouse and it had landed on my hand. I was willing to find more insects but they were being stubborn and it was almost as if the bug wanted to be caught!
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I was confident throughout the process of this project that my insect would be infected with Wolbachia due to the known key facts where my insects habitat is as well as what it intakes. It is common for “love bugs” to be present with Wolbachia. The controls did work in the process and I can definitely trust my results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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