MACACDF2319 Lepidoptera

November 7, 2023

Sample information
Picture
Photos by: Chase B.
Location No map location data available.
Collection date 08/22/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Found in a shrubby, bushy area location on the Georgia Southern Armstrong Campus. Captured using a sweeping net.
Putative identification Arthropoda Hexapoda Insecta Lepidoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis control positive, negative and NTC controls all came back properly concluding that the gel electrophoresis was most likely accurate enough to conclude a high confidence level on the outcome of the Wolbachia testing.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Lepidoptera was found to be negative for Wolbachia.
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