MAC.ACI.F23-4 HEMIPTERA

November 8, 2023

Sample information
Picture
Photos by: Julia W
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Tall grassy field in the Science Center. The weather was 86 degrees and partly cloudy at 1:15 pm and used a sweep net to collect.
Putative identification Arthropoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents were used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

The gel electrophoresis supported the fact that my insect was negative for Wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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