Sample information |
|
Picture |
|
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Location | |
Collection date | 08/23/2023 |
Captive / Cultivated? | Wild-caught |
Group | Georgia Southern University |
Observations | Moist tall grass in humid weather Collected insect the specimen with a sweep net |
Putative identification | Arthropoda Hexapoda |
Methods |
|
Extraction kit | |
DNA extraction location | Abdomen |
Single or Duplex PCR | Single Reaction |
Gel electrophoresis system | Standard electrophoresis system |
Buffer | TAE |
DNA stain | Other |
Gel images |
|
Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
Wolbachia presence | No |
Confidence level | Medium |
Explanation of confidence level | I’m confident that the controls worked. I double-checked everything and was extra careful when performing this lab. |
Wolbachia 16S sequence | |
Arthropod COI sequence |
|
Summary | The Hexapoda was found to be negative for Wolbachia. |