MAC.ACI.F23-2 Diptera

November 8, 2023

Sample information
Picture
Photos by: Abigail M.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • Date: 8/23/23​
  • Time: 1:00 PM-1:30 PM​
  • Location: Corner of Jenkins Hall and The Science Center​
  • Environment: GSU Vegetation, 86º F, Windy and Partly Cloudy​
  • Collection Method: Sweeping Vegetation using a Net​
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My controls displayed the expected outcomes therefore I am confident in my results that my insect does not have wolbachia.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Diptera was found to be negative for Wolbachia.
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