Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 09/06/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection Notes Date of Collection- August 23, 2023 Time of Collection- 1:01pm – 1:59pm Location of Collection- Savannah, Ga North of Armstrong campus parking lot Environment of Collection- Parking lot islands and tall grass right side of parking lot Method of Collection- Used mat and beat stick in the parking lot island, swung net over tall grass, and used forceps to collect insects off mat |
| Putative identification | Arthropoda Insecta Hymenoptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The results of my positive, negative, and no template control all went as they were supposed to. Which makes me confident that my results from the gel electrophoresis was successful. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hymenoptera was found to be postive for Wolbachia. |
Common Eastern Bumble Bee (Bombus impatiens)
American Bird
Spotted crane fly
Wolbachia data
Meadow Katydid