MAC.ACI.F23.#3 Hemiptera

November 8, 2023

Sample information
Picture
Photos by: Ellie T.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collected using a sweep net in a grassy field. The weather conditions were windy, sunny, and hot at the tome of collection. The insect was collected at 1:20 PM on 8/23/23.
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

I am confident in my results because the gel electrophoresis was preformed correctly because both the positive and negative controls were present. Also, the majority of the positive infections in my class were also Hemiptera.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be postive for Wolbachia.
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