Sample information |
|
| Picture |
|
|---|---|
| Location | |
| Collection date | 08/23/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collected using a sweep net in a grassy field. The weather conditions were windy, sunny, and hot at the tome of collection. The insect was collected at 1:20 PM on 8/23/23. |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | I am confident in my results because the gel electrophoresis was preformed correctly because both the positive and negative controls were present. Also, the majority of the positive infections in my class were also Hemiptera. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be postive for Wolbachia. |
Water Boatman