LOGACEF23-IA Orthoptera

November 8, 2023

Sample information
Picture
Photos by: Iona A.
Location
Collection date 08/23/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Caught with an Arial/ butterfly net and captured into a jar from a field of Tallgrass at 85 degrees of sunny but partly sunny skies.
Putative identification Arthropoda Insecta Orthoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes
  • Column 1: positive control
  • Colum 2: negative control
  • Column 3: no template control
  • Column 4: another specimen
  • Column 5: LOGACEF23-IA
  • Column 6: Ladder

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

DNA has good quality ratio of 2.05. Looking at column 5 with LOGACEF23-IA specimen, has one visible fragment, meaning it is negative.
Wolbachia 16S sequence
TCATACCTATTCGAAGGGATAGGGTCGGTTCGGCCGGATTTTACACAAGTGTT GCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACG AGCGCAACCCTCATCCTTAGTTGCCATCAGGTAATGCTGAGTACTTTAAGGAA ACTGCCAGTGATAAGCTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGC CTTTATGGAGTGGGCTACACACGTGCTACAATGGTGTCTACAATGGGTTGCA AGGTGCGCAAGCCTAAGCTAATCCCCAAAAGACATCTCAGTTCGGATTGTAC TCTGCAACTCGAGTACATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCAT GCCACGGTGAATACGT
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Summary The Orthoptera was found to be negative for Wolbachia.
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