LOGACGF23-KM-Hemiptera

November 13, 2023

Sample information
Picture
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
  • time: 6:03 pm
  • climate: humid, hot, and sunny
  • environment: marsh-like aquatic habitat
  • location: Georgia Southen University campus by University Hall
  • method: aquatic D-net
Putative identification Arthropoda Hexapoda Insecta Hemiptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

My gel electrophoresis was successful and my extraction and analyzing were precise and correct.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hemiptera was found to be negative for Wolbachia.
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