Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/22/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | Collection method: sweeping net Collection habitat: grass Weather conditions: 84 degrees, partly cloudy, slight breeze |
| Putative identification | Arthropoda Hexapoda Insecta Hemiptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | Based on my articles, local, and class prevalence, as well as the gel electrophoresis results, I am very confident that my controls worked properly and that I do not have a false positive. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Hemiptera was found to be postive for Wolbachia. |
Water Boatman