Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | 08/21/2023 |
| Captive / Cultivated? | Wild-caught |
| Group | Georgia Southern University |
| Observations | One pair of developed wings Length of wings are the same as abdomen Maxillary Palps and Labial for mouth |
| Putative identification | Arthropoda Hexapoda Insecta Diptera |
Methods |
|
| Extraction kit | |
| DNA extraction location | Abdomen |
| Single or Duplex PCR | Single Reaction |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TAE |
| DNA stain | Other |
| Gel images |
|
| Protocol notes | DNA extraction kit of in-house reagents was used |
Results |
|
| Wolbachia presence | No |
| Confidence level | High |
| Explanation of confidence level | Only one bp band appeared for my sample. The CO1 band |
| Wolbachia 16S sequence |
Bp 438
BLAST at The Wolbachia Project BLAST at NCBI
|
| Arthropod COI sequence |
Bp 708
BLAST at The Wolbachia Project BLAST at NCBI
|
| Summary | The Diptera was found to be negative for Wolbachia. |
Water Boatman