LOGACGF23KMS-Diptera

November 13, 2023

Sample information
Picture
Photos by: Kimberly S.
Location No map location data available.
Collection date 08/21/2023
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

One pair of developed wings

Length of wings are the same as abdomen

Maxillary Palps and Labial for mouth
Putative identification Arthropoda Hexapoda Insecta Diptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

Only one bp band appeared for my sample. The CO1 band
Wolbachia 16S sequence
Bp 438
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence
Bp 708
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Diptera was found to be negative for Wolbachia.
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