Phytomyzinae

December 7, 2023

Sample information
Picture
Photos by: Elio E., Salome G.
Location
Collection date 11/09/2023
Captive / Cultivated? Wild-caught
Group Gymnasium Immensee
Observations

Caught under a tree while flying. Little forest next to a school building. Around 14 degrees celsius and drizzle rain. Not sure if Phytomyzinae.
Putative identification Arthropoda Insecta Diptera Agromyzidae

Methods
Extraction kit Edwards Buffer
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes
  1. Phytomyzinae
  2. Control: definitive Wolbachia positive arthropod
  3. Control: definitive Wolbachia positive DNA

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

Worked under sterile circumstances. Results look promising with two definite Wolbachia-positive probes.

bp n seems to be exactly right. 438bp for Wolbachia and 708bp for COI.

(We are confident workers :). )
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Agromyzidae was found to be postive for Wolbachia.
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