Oniscus asellus

December 7, 2023

Sample information
Picture
Photos by: Floyd Sieben, Maximilian Schmitt, Felix Lüthold
Location
Collection date 11/09/2023
Captive / Cultivated? Wild-caught
Group Gymnasium Immensee
Observations

Wet old tree trunk. It was relatively cold and very wet. It was around the afternoon when it was found.

 
Putative identification Arthropoda Crustacea Malacostraca Isopoda Oniscidae Oniscus Oniscus asellus

Methods
Extraction kit BABEC
DNA extraction location Partial abdomen
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

Lane 7 is the Oniscus asellus

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We worked through the diffrent steps carefully.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Oniscus asellus was found to be negative for Wolbachia.
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