Musca Domestica, 6405 Immensee (CH)

December 7, 2023

Sample information
Picture
Photos by: Felix L., Floyd S., Maximilian S.
Location
Collection date 11/09/2023
Captive / Cultivated? Wild-caught
Group Gymnasium Immensee
Observations

Weather: High humidity, no rain, cloudy, wet

Location: Underneath the ceiling of a bus stop
Putative identification Arthropoda Hexapoda Insecta Diptera Muscidae Musca Musca domestica

Methods
Extraction kit BABEC
DNA extraction location Whole arthropod
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system MiniOne
Buffer TAE
DNA stain GelGreen
Gel images
Protocol notes

On the Gel image, the respective lane is the fourth from the left (we were unable to extract DNA, so there’s no colour).

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

We worked through the different steps carefully.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Musca domestica was found to be negative for Wolbachia.
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