Sample information |
|
| Picture |
|
|---|---|
| Location | No map location data available. |
| Collection date | |
| Captive / Cultivated? | |
| Group | Antioch High School |
| Observations | |
| Putative identification | Arthropoda |
Methods |
|
| Extraction kit | |
| DNA extraction location | |
| Single or Duplex PCR | |
| Gel electrophoresis system | Standard electrophoresis system |
| Buffer | TBE |
| DNA stain | SYBR Safe |
| Gel images |
|
| Protocol notes | we made a small error contaminated the test tube, didn’t run electrophoresis long enough. |
Results |
|
| Wolbachia presence | No |
| Confidence level | Low |
| Explanation of confidence level | we’re not very confident because of everything that went on during the lab that we describe in the protocol notes like how e dropped out test tube. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Arthropoda was found to be negative for Wolbachia. |
Anyphaena accentuata NP1
Cylindroiulus caeruleocinctus NP2
Lithobius forficatus SM1
Oniscus asellus GC1
Oniscus Asellus (LS1)