Trachelipus ratzeburgii YC2

May 8, 2024

Sample information
Picture
Photos by: Yara C.
Location
Collection date 03/07/2024
Captive / Cultivated? Wild-caught
Group KantiWil
Observations

I don’t have a photo, but i caught the insect in a small forest near my school. I caught it at 8 o’clock in the morning and the temperature was about 3 degrees. It was under a pile of leaves. Only this insect and no others were sighted in this place.
Putative identification Arthropoda Malacostraca Isopoda Trachelipodidae Trachelipus Trachelipus ratzeburgii

Methods
Extraction kit Instagene Matrix
DNA extraction location Rear half
Single or Duplex PCR Duplex Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain SYBR Safe
Gel images
Protocol notes

Results
Wolbachia presence Yes
Confidence level High
Explanation of confidence level

 

The result is trustworthy, as all work steps were carried out properly and the DNA duplex worked. In YC2 you can see that a band appeared at 438bp. Therefore you can say that YC2 is a Wolbachia carrier. One band appeared at 708bp. This says that YC1 is an arthropod.
Wolbachia 16S sequence Download FASTA    Download AB1
TGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTCATCCTTAGTTACCATCAGATAATGCTGGGGACTTTAAGGAAACTGCTAGTGATAAACTGGAGGAAGGTGGGGATGATGTCAAGTCATCATGGCCCTTATGGAGTGGGCTACACACGTGCTACAATGGTGGCTACAATGGGCTGCAAAGTCGCGAGGCTAAGCTAATCCCTTAAAAGCCATCTCAGTTCGGATTGCACTCTGCAACTCGAGTGCATGAAGTTGGAATCGCTAGTAATCGTGGATCAGCATGCCACGGTGAATACGTTCTCGGGTCTTGTACACACTGCCCGTCACGCCATGGGAATTGGTTTCCTCGAAGCTGTCCCTTCGAATAGGTATGAGGAGCCCC
BLAST at The Wolbachia Project   BLAST at NCBI
Arthropod COI sequence Download FASTA    Download AB1
CCWCGCTCAGGATGATTATTCGAACAGAGCTAGGTTCCCCTGGTAGATTAATTGGGGACGACCAGATTTACAATGTTATTGTTACAGCACATGCTTTTGTTATAATTTTCTTTATAGTAATACCTATTATAATTGGAGGTTTTGGTAATTGATTAGTTCCGTTAATATTAGGGGCTCCTGACATAGCATTCCCGCGTATAAATAATATAAGATTTTGGCTTCTCCCACCTTCTTTAGTTCTTCTCCTTAGAAGAGGGTTGATTGAAAGGGGAGTAGGGACTGGCTGAACTGTTTACCCTCCGTTGGCTGGCGGAATTGCTCACAGAGGGGCTTCAGTAGATTTAGGAATTTTTTCTCTTCATCTTGCGGGGGCTTCTTCAATTTTAGGTGCAGTAAATTTTATTACTACTGTAGCTAATATACGTTCTCCTGGTATAAAAATAGATCGGGTGCCTTTATTTGTATGGTCTGTTTTAATTACAGCTGTTTTATTATTACTTTCGTTACCTGTTTTAGCAGGCGCTATTACTATGTTGCTAACTGACCGTAATTTAAATACATCTTTTTTTGATCCTAGCGGGGGAGGAGACCCTATTTTATACCAGCATTTATTTTGATTTTTTGGTCACCTKGAAAGTTTAAAA
BLAST at The Wolbachia Project   BLAST at NCBI
Summary The Trachelipus ratzeburgii was found to be postive for Wolbachia.
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