DEMAASP24-7; Hymenoptera

April 8, 2024

Sample information
Picture
Photos by: Catherine D.
Location
Collection date 01/22/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations

Collection date: 01/22/2024

Collection time: 1:10 pm

Collection method: plastic plate, tweezers

Collection habitat: ant hill

Weather conditions: 55 degrees Fahrenheit; partly cloudy
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Whole arthropod
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents was used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

All controls had the expected results, therefore I am highly confident that the PCR worked as expected and there was no cross contamination.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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