Fungus Gnat 3-3 Remote

Sample information
Picture	Photos by: Kabir M.
Location
Collection date	10/09/2020
Captive / Cultivated?	Wild-caught
Group	Acton-Boxborough Regional High School
Observations	The fungus gnat was collected/found in a patch of grass in between mowed and unmowed lawn, so there were some well-maintained lawn and some dryer, yellow grass with weeds. There were also very few leaves in the area, and it was fairly open. There was also mulch a few feet away from the area. It was around 10:00 AM when the gnat was collected. It was about 65º F and cloudy.
Putative identification	Arthropoda Insecta Diptera Mycetophilidae
Methods
Extraction kit	DNeasy (Qiagen)
DNA extraction location	Abdomen
Single or Duplex PCR
Gel electrophoresis system	MiniOne
Buffer	TBE
DNA stain	GelGreen
Gel images
Protocol notes
Results
Wolbachia presence	No
Confidence level	High
Explanation of confidence level	The controls worked and were CO1 positive, which validates the quality of the wolbachia negative.
Wolbachia 16S sequence
Arthropod COI sequence	Download AB1
Summary	The Mycetophilidae was found to be negative for Wolbachia.

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The Wolbachia Project Database