|Captive / Cultivated?||Wild-caught|
|Group||St. John High School USD 350|
The flea was captured off of my dog in mid September. He had been under some trees.
|Putative identification||Hexapoda Insecta Siphoneptera|
|Extraction kit||DNeasy (Qiagen)|
|DNA extraction location||Whole arthropod|
|Single or Duplex PCR|
|Gel electrophoresis system||MiniPCR|
The flea was stored in 90% ethanol for about two months prior to extraction.
DNA Extraction: The DNA was extracted from the whole arthropod. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete.
PCR: Mini One Taq polymerase was used.
Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear.
|Explanation of confidence level||
The flea had two bright bands that matched up with my positive controls and all the of the protocols were followed exactly. I’m confident the flea is positive for Wolbachia.
|Wolbachia 16S sequence||
|Arthropod COI sequence||
|Summary||The Siphoneptera was found to be postive for Wolbachia.|