Sample information |
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| Picture |
|
|---|---|
| Location | |
| Collection date | 09/16/2020 |
| Captive / Cultivated? | Wild-caught |
| Group | St. John High School USD 350 |
| Observations | The flea was captured off of my dog in mid September. He had been under some trees. |
| Putative identification | Arthropoda Hexapoda Insecta Siphoneptera |
Methods |
|
| Extraction kit | DNeasy (Qiagen) |
| DNA extraction location | Whole arthropod |
| Single or Duplex PCR | |
| Gel electrophoresis system | MiniPCR |
| Buffer | TBE |
| DNA stain | GelGreen |
| Gel images |
|
| Protocol notes | The flea was stored in 90% ethanol for about two months prior to extraction. DNA Extraction: The DNA was extracted from the whole arthropod. All of the Dneasy (Qiagen) protocols were followed. The extraction took two days to complete. PCR: Mini One Taq polymerase was used. Gel Electrophoresis: Mini PCR was used. All of the lanes worked well and were bright and clear. |
Results |
|
| Wolbachia presence | Yes |
| Confidence level | High |
| Explanation of confidence level | The flea had two bright bands that matched up with my positive controls and all the of the protocols were followed exactly. I’m confident the flea is positive for Wolbachia. |
| Wolbachia 16S sequence | |
| Arthropod COI sequence |
|
| Summary | The Siphoneptera was found to be postive for Wolbachia. |
Water Boatman