LOGADS24-NM Hymenoptera

April 9, 2024

Sample information
Picture
Photos by: Nicole M.
Location
Collection date 01/23/2024
Captive / Cultivated? Wild-caught
Group Georgia Southern University
Observations
•Time of Collection: 11:57am
In the tall grass bush in front of Lane Library on the Armstrong Campus (GSU)
Method of Collection: Sweeping Net
Putative identification Arthropoda Hexapoda Insecta Hymenoptera

Methods
Extraction kit
DNA extraction location Abdomen
Single or Duplex PCR Single Reaction
Gel electrophoresis system Standard electrophoresis system
Buffer TAE
DNA stain Other
Gel images
Protocol notes

DNA extraction kit of in-house reagents were used.

Results
Wolbachia presence No
Confidence level High
Explanation of confidence level

I saw that all my results looked correct compared to other samples, and compared them to people with the same order as mine with the same outlooked results.
Wolbachia 16S sequence
Arthropod COI sequence
Summary The Hymenoptera was found to be negative for Wolbachia.
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